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therefore, three strains of Lactobacillus , each shown alone to have a protective effect against the development of EAE when administered before disease onset, were shown to inhibit established disease when administered as a therapeutic mixture

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Beads were sequentially washed in 500-l solutions of: 1x 1% SDS in 50 mM HEPES, 1x 500 l 0.2% SDS in 50 mM HEPES, 1x 500 l 0.2% SDS, 40 mM CAA, 10 mM TCEP in 50 mM HEPES (10-min incubation with agitation) and finally 2x 500 l 50 mM HEPES

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*Correspondence: Cesare Indiveri, [email protected] This article was submitted to Molecular and Cellular Oncology, a section of the journal Frontiers in Cell and Developmental Biology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers