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The brain may begin producing less dopamine naturally, making individuals feel fatigued, depressed, or unmotivated when the drug is not present

14 Resulting cell suspensions were centrifuged (5 min, 300 g ) and the pellets were resuspended in DMEM (supplemented with 10% fetal calf serum and 1% antibiotics, 100 U ml 1 penicillin and 0.1 mg ml 1 streptomycin)

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The PDS develops from large and relatively slow excitatory postsynaptic potentials mediated by AMPA and NMDA glutamate receptors activated by aberrant and large glutamate release within the foci